The demand of resources and fuels for the technological development is increasing day by day. In order to keep the pace of development high we need to think about some alternate fuel which would help overcome the demand keeping in mind the resources for the future generation. An alternate fuel needs to be developed and researched upon which could help us get a greener and better tomorrow. In this study the experimental tests on a single cylinder diesel engine using esterified rubber seed oil bleeds with diesel are conducted. The performances parameters thermal efficiency (BTE) and brake specific fuel consumption (BSFC) are analyzed with different inlet pressures (180 bar, 200 bar, 220 bar) and various load conditions. Also emission analysis of hydrocarbons (HC) and Nitric Oxide (NOx) has been carried out. It was found that the thermal efficiency of engine is increased at the pressure of 220 bar and considerable reduction of HC, NOx was absorbed.