Work-life balance is a contemporary issue that is gaining increasing attention from all the stakeholders, and is in the fore front of board room discussions. Change in the work force demographics and work culture, advancement in the field of communication and information technology are some of the factors that influence work-life balance. The Erosion of male bread winner model and the change in the values and attitude of the current generation work force makes it necessary to address the work-life balance issue. In BPO Industry, like men, women equally participate in the shift work, including night shift. A survey is conducted among women employed in BPO organizations to study the influence of shift work on work-life balance. Relationship between shift work and work-life conflict, life-work conflict, psychosomatic disorders and turnover intentions are studied. The findings of the study suggest that respondents working in non-standard working hours (working in second shift and night shift) had better work-life balance. Work-life conflict was found to be low for respondents working in second shifts and incidence of psychosomatic disorders was low for the respondents working in night shifts influence of shift work on work-life balance, work-life conflict, life-work conflict, psychosomatic disorders and turnover intentions.