The fast depletion of Petroleum fuels and increasing costs concerns have led to an intensive search for alternative fuels such as biodiesel. In this experimental study a dual fuel includes rubber seed oil and jatropha oil blended with the pure diesel fuel was used. The dual fuel was estrified to reduce the viscosity and it was blended with the diesel fuel for the different proportions like 20%, 40%, 60% of biodiesel blended with diesel namely B20, B40, and B60. The performance and emission test was carried out in a single cylinder constant speed (1500 rpm) direct injection (Dl) diesel engine. In this engine the injection timing was varied to study the performance and emissions from biodiesel fuelled diesel engine. The experiment was performed at the injection timing of 27 BTDC and 220 bar of injection pressure with reference to the standard injection timing of 24 BTDC and the normal injection pressure. The performance test resulted with the increased Brake thermal efficiency (BTE) and reduction in the specific fuel energy consumption (SFEC) for B20 blend as compared to the other proportions. From the emission characteristics results the carbon monoxide (CO), unburned hydrocarbon (UHC), oxides of nitrogen (NOx) and carbon dioxide (CO2) were decreased for B20 when compared with the other proportions.